人類自然殺手細胞冷凍保存技術

細胞生物製劑產品與關鍵原料開發驗證計畫

自然殺手細胞(NK cells)具強大抗癌潛力，然而，NK 細胞對冷凍耐受性極低，解凍後常面臨存活率下降與活性受損，導致皆以活化製備為主，造成高昂成本與捐贈者反覆採樣的負擔。本技術建立一套簡單、安全且具經濟效益的製程，能有效減緩冷凍對 NK 細胞的生理損傷，解決活性流失難題，是推動細胞產品量產化與普及化的關鍵製劑技術。

Natural Killer cells (NK cells) are unique cells in the human immune system. However, compared to freshly isolated and highly activated cells, cryopreserved NK cells generally exhibit reduced viability, insufficient recovery, impaired cytokine secretion, and decreased cytotoxic activity after thawing, which significantly limits their clinical efficacy. In this work, we developed a simple, safe, and cost-effective strategy to preserve NK cell function after cryopreservation, establishing it as a key enabling technology for the development of NK cell–based therapeutic products.